One Step Tropical Diseases LEP Leptospira IgM/IgG Antibodies Rapid Diagnostic Blood Screening Test Kit
Introduction of LEP:
Leptospirosis is an infectious disease caused by Leptospira spp. There are at least four species of the leptospira bacteria (orserovars) that can infect dogs: canicola, icterohemorrhagiae, grippotyphosa, and pomona.The severity of symptoms varies, and depends on the dog (age, immune response, vaccination status), the strain of Leptospira, and other factors. Some dogs may have mild symptoms or no symptoms at all, but severe cases can be fatal.Signs appear 4 to 12 days after exposure. In early stage, fever is present. Other signs are loss of appetite for several days, vomiting , lethargy, depression, muscle pain, and sometimes diarrhea or blood in the urine.
Functions of LEP:
Detection of Canine LeptospiraIgMantibody from Canine whole blood, serum or plasma.
Principle of LEP:
Leptospira IgM Ab Test Kit is designed to detect Leptospira IgM antibodies in canine whole blood, serum or plasma.After being absorbed into the cellulose pad,the antibodies of Leptospira move and bind with gold-colloidcomplex. This complex is binding test line in the nitrocellulose membrane. The test results can appear on Control (C) and Test (T) lines where the principles of immunochromatography are used.
Characteristics of LEP:
1.One-step rapid test of Canine Leptospira IgM antibody
2.Rapid test results between 5 ~ 10 minutes
3.Expensive equipment not required
4.Easy storage and maintenance 5.High-purity and high-quality materials of the test kit increase its sensitivity and specificity.
The test kits stored at 2~30℃ can be used for 24 months after manufacturing. Do not keep them in a refrigerator. However, if they are stored under cold circumstances, keep them at room temperature for 15 ~ 30 minutes before use.
Interpretation of the results:
1.A purple band should appear on the control line regardless of the test result. The presence of another band on the test line determines the result. Control Line (C): The line should always appear regardless of the presence of the antibodies of Leptospira. If this line does not appear, the test should be considered invalid. This might be because of impure buffer or the lack of specimen. It should be tested again with another kit. Test Line (T): The presence of IgMantibodiesof Leptospira determines the test line. 2.LeptospiraIgM Positive (+): A positive result implies that thedog has the anti-LeptospiraIgM. The dog is in an initial infection phase or in non-suspected dogs may occur as a result from previous vaccination or acute but sub-clinical infection. LeptospiraIgMNegative (-): A negative result implies that thedog has no anti-LeptopsriaIgM. If the dog is suspected to be infected but has negative result, it needs to be tested repeatedly 2 or 3 times more. The time of the test could have been the period of seroconversion. 3.Further examinations: This test is for primary screening only. Consult veterinarians for further necessary examinations to obtain clinical test results.
1.Use for Caninein-vitro diagnostic purposes only. 2.Use within 10 minutes after opening the pouch because the test kit is very sensitive to moisture and its effect may diminish. 3.Be careful of not touching the result window. 4.Every specimen should be used with different droppers. 5.For testing, the buffer included should be used. 6.Do not use specimen showing hemolysis or being contaminated with microbes,which may cause falsepositive or false negative result. 7.Deal with specimen carefully. They can deliver unknown virus or infectious bacteria. 8.Use disposable gloves when you suspect the infection caused by specimen. And wash your hands later. 9.Dispose solid wastes after sterilizing them at 121℃ for over 1 hour. 10.Do not use the kit when its pouch is torn, sealing is not good in shape or expiration date is passed.
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