Pathological Analysis Equipments Veterinary diagnostic Simple and rapid field tests for brucellosis in livestock

Veterinary diagnostic Simple and rapid field tests for brucellosis in livestock

Introduction

Rapid Brucellosis (BSP) antigen rapid test kit using rapid immunochromatographic test for the detection of brucellosis antigens. After the sample to be added to the loading hole, it moves along the chromatographic membrane together with the colloidal gold labeled anti-BSP monoclonal antibody. When a BSP antigen is present in the sample, it shows a red-wine color by binding to the antibody on the assay line. No color reaction occurs if no BSP antigen is present in the sample.

Contents

Procedure

1. Preparation of sample test solution:

A. Pure culture bacteria: pick a single suspected brucella colonies in the sample buffer, cover the tube cover violent concussion more than 30 seconds, and then allowed to stand for 5 minutes, to be supernatant after the precipitation of large particles as a detection solution .

B.Clinical specimens: with swab dipped in suspected brucellosis of the inguinal lymph node acupuncture aspirates, vaginal discharge, diseased tissue homogenates. Immediately insert the swab into the test tube containing the sample buffer. dissolve the sample then stand for 5 minutes until the large particles precipitated supernatant as a test solution.

C.Milk: Collect 20ml raw milk, centrifuge for 15 minutes at 2000g, discard the supernatant, dip a centrifuge tube sediment with swab, then immediately insert the cotton swab into the sample buffer, place the swab in the test tube Repeatedly force the wall rotation at least 10 times, and mix the solution.

2. Remove the test device from the foil pouch, and place it on a flat and dry surface.

3. Take 5 drops of sample test solution to the test device sample well(S mark).

4. At this time will see the wine red liquid flow through the observation window. Read test result in 15-20minutes , results will invalid after 20 minutes..

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